Extensive regulation of nicotinate phosphoribosyltransferase (NAPRT) expression in human tissues and tumors

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Duarte Pereira, S (Author) Other; Pereira Castro, I (Author) Other; Silva, SS (Author) Other; Correia, MG (Author) Other; Neto, C (Author) Other; da Costa, LT (Author) Other; Amorim, A (Author) FCUP; Silva, RM (Author) Other

Journal

Title: Oncotarget

Vol. 7

Pages: 1973-1983

ISSN: 1949-2553

Publisher: Impact Journals

Indexing

ISI Web of Knowledge - 46 Citations

Scopus - 45 Citations

Other information

Authenticus ID: P-00K-5YQ

DOI: 10.18632/oncotarget.6538

Abstract (EN): Nicotinamide adenine dinucleotide (NAD) is a cofactor in redox reactions and a substrate for NAD-consuming enzymes, such as PARPs and sirtuins. As cancer cells have increased NAD requirements, the main NAD salvage enzymes in humans, nicotinamide phosphoribosyltransferase (NAMPT) and nicotinate phosphoribosyltransferase (NAPRT), are involved in the development of novel anticancer therapies. Knowledge of the expression patterns of both genes in tissues and tumors is critical for the use of nicotinic acid (NA) as cytoprotective in therapies using NAMPT inhibitors. Herein, we provide a comprehensive study of NAPRT and NAMPT expression across human tissues and tumor cell lines. We show that both genes are widely expressed under normal conditions and describe the occurrence of novel NAPRT transcripts. Also, we explore some of the NAPRT gene expression mechanisms. Our findings underline that the efficiency of NA in treatments with NAMPT inhibitors is dependent on the knowledge of the expression profiles and regulation of both NAMPT and NAPRT.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11