Epidemiological Cutoff Values for Fluconazole, Itraconazole, Posaconazole, and Voriconazole for Six Candida Species as Determined by the Colorimetric Sensititre YeastOne Method

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Canton, E (Author) Other; Peman, J (Author) Other; Iniguez, C (Author) Other; Hervas, D (Author) Other; Lopez Hontangas, JL (Author) Other; pina-vaz, c (Author) FMUP; Camarena, JJ (Author) Other; Campos Herrero, I (Author) Other; Garcia Garcia, I (Author) Other; Garcia Tapia, AM (Author) Other; Guna, R (Author) Other; Merino, P (Author) Other; Perez del Molino, LP (Author) Other; Rubio, C (Author) Other; Suarez, A (Author) Other

Journal

Title: Journal of Clinical Microbiology

Vol. 51

Pages: 2691-2695

ISSN: 0095-1137

Publisher: American Society for Microbiology

Indexing

ISI Web of Knowledge - 32 Citations

Scopus - 34 Citations

Other information

Authenticus ID: P-006-8SF

DOI: 10.1128/jcm.01230-13

Abstract (EN): In the absence of clinical breakpoints (CBP), epidemiological cutoff values (ECVs) are useful to separate wild-type (WT) isolates (without mechanisms of resistance) from non-WT isolates (those that can harbor some resistance mechanisms), which is the goal of susceptibility tests. Sensititre YeastOne (SYO) is a widely used method to determine susceptibility of Candida spp. to antifungal agents. The CLSI CBP have been established, but not for the SYO method. The ECVs for four azoles, obtained using MIC distributions determined by the SYO method, were calculated via five methods (three statistical methods and based on the MIC50 and modal MIC). Respectively, the median ECVs (in mg/liter) of the five methods for fluconazole, itraconazole, posaconazole, and voriconazole (in parentheses: the percentage of isolates inhibited by MICs equal to or less than the ECVs; the number of isolates tested) were as follows: 2 (94.4%; 944), 0.5 (96.7%; 942), 0.25 (97.6%; 673), and 0.06 (96.7%; 849) for Candida albicans; 4 (86.1%; 642), 0.5 (99.4%; 642), 0.12 (93.9%; 392), and 0.06 (86.9%; 559) for C. parapsilosis; 8 (94.9%; 175), 1 (93.7%; 175), 2 (93.6%; 125), and 0.25 (90.4%; 167) for C. tropicalis; 128 (98.6%; 212), 4 (95.8%; 212), 4 (96.0%; 173), and 2 (98.5; 205) for C. glabrata; 256 (100%; 53), 1 (98.1%; 53), 1 (100%; 33), and 1 (97.9%; 48) for C. krusei; 4 (89.2%; 93), 0.5 (100%; 93), 0.25 (100%; 33), and 0.06 (87.7%; 73) for C. orthopsilosis. All methods included >= 94% of isolates and yielded similar ECVs (within 1 dilution). These ECVs would be suitable for monitoring emergence of isolates with reduced susceptibility by using the SYO method.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5