Poly-A-rich domains in adrenal cell nuclear matrix after acute and chronic ACTH stimulation: an in situ hybridization study

• Type: Article in International Scientific Journal
• Date: 2000

Authors

D Neves (Author) FMUP; Magalhaes, MM (Author) Other; Magalhaes, MC (Author) Other

Journal

Title: Cell and Tissue Research

Vol. 302

Pages: 81-84

ISSN: 0302-766X

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-000-YSV

DOI: 10.1007/s004410000255

Abstract (EN): The regulatory effects of adrenocorticotropin (ACTH) at the adrenal cell nucleus are not yet completely clarified. Recent studies showed that eukaryotic cell nucleus is composed of specific subcompartments organized by the nucleoskeleton-nuclear matrix. In this paper, the RNA polymerase II transcriptional domains were detected in the adrenal nucleus after acute and chronic ACTH stimulation by using in situ hybridization. Polydeoxythymidine (poly-T) probe, complementary to the polyadenylated (poly-A) chain of all the mRNAs and its precursors, was used. Hybridization was performed in intact adrenal cells and in nuclear matrices isolated from previously dispersed cells. The transcriptional domains, enriched in poly-A RNA, were evident in intact adrenal cells, the staining intensity being higher both in the acutely and in the chronically stimulated animals. In the nuclear matrices poly-A-rich areas were still visible, and were more intensely stained after ACTH stimulation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4