Expression of the ETV6-NTRK3 gene fusion as a primary event in human secretory breast carcinoma

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Tognon, C (Author) Other; Knezevich, SR (Author) Other; Huntsman, D (Author) Other; Roskelley, CD (Author) Other; Melnyk, N (Author) Other; Mathers, JA (Author) Other; Becker, L (Author) Other; Carneiro F (Author) FMUP; MacPherson, N (Author) Other; Horsman, D (Author) Other; Poremba, C (Author) Other; Sorensen, PHB (Author) Other

Journal

Title: Cancer Cell

Vol. 2

Pages: 367-376

ISSN: 1535-6108

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 584 Citations

Scopus - 640 Citations

Other information

Authenticus ID: P-000-MFV

DOI: 10.1016/s1535-6108(02)00180-0

Abstract (EN): We report that human secretory breast carcinoma (SBC), a rare subtype of infiltrating ductal carcinoma, expresses the ETV6-NTRK3 gene fusion previously cloned in pediatric mesenchymal cancers. This gene fusion encodes a chimeric tyrosine kinase with potent transforming activity in fibroblasts. ETV6-NTRK3 expression was confirmed in 12 (92%) of 13 SBC cases, but not in other ductal carcinomas. Retroviral transfer of ETV6-NTRK3 (EN) into murine mammary epithelial cells resulted in transformed cells that readily formed tumors in nude mice. Phenotypically, tumors produced glands and expressed epithelial antigens, confirming that EN transformation is compatible with epithelial differentiation. This represents a recurrent chromosomal rearrangement and expression of a dominantly acting oncogene as a primary event in human breast carcinoma.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10