Cultures of kidney transplant fine-needle aspiration samples from rejection-free patients produce a specific antidonor response suppressive factor

• Type: Article in International Scientific Journal
• Date: 2002

Authors

José Oliveira (Author) FMUP; Xavier, P (Author) Other; Ramos, J (Author) Other; Sampaio, S (Author) Other; Magalhaes, M (Author) Other; Mendes, A (Author) Other; Pestana M (Author) FMUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: NEPHRON

Vol. 91

Pages: 637-645

ISSN: 0028-2766

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-000-NHZ

DOI: 10.1159/000065025

Abstract (EN): Background/Aims: Growth of T cell lines from kidney graft biopsy specimens that suppress the antidonor response, either directly or through a soluble factor, both specific and nonspecific to donor, has been reported. Fine-needle aspiration biopsy sample cultures synthesize a large array of cytokines/chemokines with significant differences between stable versus acute rejection transplants. We hypothesize that the products of such cultures may be endowed with antidonor response modulation abilities in kidney transplantation. Methods: From 46 patients, 21 rejection free (group A) and 25 developing 28 acute rejection crises (group B), samples were obtained on days 7 and 30 after transplantation in group A and on day 7 and on acute rejection day in group B. The supernatants obtained after 48 h of culture were studied for IL-1 receptor antagonist, IL-4, IL-4 soluble receptor alpha, IL-12, IL-13, IFN-gamma, TGF-beta1, TGF-beta2, GM-CSF, and PGE(2) and for their effects on mixed lymphocyte reactions, donor-recipient and recipient-third-party combinations. At the end, analysis by flow cytometry of donor-recipient cultures complemented the analysis done before cultures. Results: Day 7 supernatants from group A specifically and significantly inhibited the antidonor response; supernatants from group B nonspecifically stimulated the antidonor response. The IL-1 receptor antagonist production was significantly higher by day 7 in group A, and the PGE(2) production was significantly higher in group B. Flow cytometry analysis did not disclose significant differences between inhibited versus stimulated antidonor responses. Conclusions: Cultures of aspiration biopsy samples done early after transplantation in rejection-free patients produce soluble suppression-specific antidonor response factor(s), not present in cultures from biopsy specimens taken before and during rejection. This was associated with IL-1 receptor antagonist synthesis. Copyright (C) 2002 S. Karger AG, Basel.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9