Comprehensive Genetic Analysis and Structural Characterization of CYP21A2 Mutations in CAH Patients

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Carvalho, B (Author) Other; Pereira, M (Author) Other; Marques, CJ (Author) FMUP; Carvalho, Davide (Author) FMUP; Leao, M (Author) Other; João Paulo Oliveira (Author) FMUP; barros, a (Author) FMUP; carvalho, f (Author) FMUP

Journal

Title: EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES

Vol. 120

Pages: 535-539

ISSN: 0947-7349

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-002-4MZ

DOI: 10.1055/s-0032-1323805

Abstract (EN): Congenital Adrenal Hyperplasia (CAH) due to 21-hydroxylase deficiency is a common autosomal recessive disorder caused by mutations in the steroid 21-hydroxylase gene (CYP21A2). Complete DNA sequencing of CYP21A2 was performed in 5 patients, 3 non-classic and 2 classic forms of the disease, that were previously screened for the 10 most common mutations, in order to detect additional mutations that could justify the phenotype of the patients. 5 mutations were identified with the whole gene extended analysis. The mutations, p.Pro432Leu and p.Ala434Glu, the first previously reported by our group and the second a novel one were structurally analyzed with ICM-Pro software regarding biochemical properties such as protein stability, accessibility to surface and hydrophobicity, in order to elucidate their effects on the CYP21A2 protein. The 2 affected residues, Pro432 and Ala434, were also studied for conservation purposes in order to predict the severity of both mutations with PolyPhen-2 software and were considered as "probably damaging". Prediction of clinical severity, based on molecular modelling and sequence conservation, was in accordance with the patient's clinical diagnosis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5