Integration of next-generation sequencing in clinical diagnostic molecular pathology laboratories for analysis of solid tumours; an expert opinion on behalf of IQN Path ASBL

• Type: Another Publication in an International Scientific Journal
• Date: 2017

Authors

Deans, ZC (Author) Other; Costa, JL (Author) Other; Cree, I (Author) Other; Dequeker, E (Author) Other; Edsjö, A (Author) Other; Henderson, S (Author) Other; Hummel, M (Author) Other; Ligtenberg, MJ (Author) Other; Loddo, M (Author) Other; Machado JC (Author) FMUP; Marchetti, A (Author) Other; Marquis, K (Author) Other; Mason, J (Author) Other; Normanno, N (Author) Other; Rouleau, E (Author) Other; Schuuring, E (Author) Other; Snelson, KM (Author) Other; Thunnissen, E (Author) Other; Tops, B (Author) Other; Williams, G (Author) Other; van Krieken, H (Author) Other; Hall, JA (Author) Other; On Behalf Of Iqn Path Asbl, (Author) Other

Journal

Title: Virchows Archiv

Vol. 470

Pages: 5-20

ISSN: 0945-6317

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 57 Citations

Scopus - 60 Citations

Other information

Authenticus ID: P-00K-X7K

DOI: 10.1007/s00428-016-2025-7

Abstract (EN): The clinical demand for mutation detection within multiple genes from a single tumour sample requires molecular diagnostic laboratories to develop rapid, high-throughput, highly sensitive, accurate and parallel testing within tight budget constraints. To meet this demand, many laboratories employ next-generation sequencing (NGS) based on small amplicons. Building on existing publications and general guidance for the clinical use of NGS and learnings from germline testing, the following guidelines establish consensus standards for somatic diagnostic testing, specifically for identifying and reporting mutations in solid tumours. These guidelines cover the testing strategy, implementation of testing within clinical service, sample requirements, data analysis and reporting of results. In conjunction with appropriate staff training and international standards for laboratory testing, these consensus standards for the use of NGS in molecular pathology of solid tumours will assist laboratories in implementing NGS in clinical services.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16