Macrophage polarization following chitosan implantation

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Vasconcelos, DP (Author) Other; Fonseca, AC (Author) Other; Costa, M (Author) ICBAS; Amaral, IF (Author) FEUP; Mário Adolfo Barbosa (Author) ICBAS; AP Águas (Author) ICBAS; Judite N Barbosa (Author) ICBAS

Journal

Title: Biomaterials

Vol. 34

Pages: 9952-9959

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 57 Citations

Scopus - 80 Citations

Other information

Authenticus ID: P-006-JBT

DOI: 10.1016/j.biomaterials.2013.09.012

Abstract (EN): Macrophages are a key cell in the host response to implants and can be polarized into different phenotypes capable of inducing both detrimental and beneficial outcomes in tissue repair and remodeling, being important in tissue engineering and regenerative medicine. The objective of this study was to evaluate the macrophage response to 3D porous chitosan (Ch) scaffolds with different degrees of acetylation (DA, 5% and 15%). The M1/M2 phenotypic polarization profile of macrophages was investigated in vivo using a rodent air-pouch model. Our results show that the DA affects the macrophage response. Ch scaffolds with DA 5% induced the adhesion of lower numbers of inflammatory cells, being the M2 the predominant phenotypic profile among the adherent macrophages. In the inflammatory exudates F4/80(+)/CD206(+) cells (M2 macrophages) appeared in higher numbers then F4/80(+)/CCR7(+) cells (M1 macrophages), in addition, lower levels of pro-inflammatory cytokines together with higher levels of anti-inflammatory cytokines were found. Ch scaffolds with DA 15% showed opposite results, since M1 were the predominant macrophages both adherent to the scaffold and in the exudates, together with high levels of pro-inflammatory cytokines. In conclusion, Ch scaffolds with DA 5% induced a benign M2 anti-inflammatory macrophage response, whereas Ch scaffolds with DA 15% caused a macrophage M1 pro-inflammatory response.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8