The attraction of Mac-1(+) phagocytes during acute inflammation by methyl-coated self-assembled monolayers

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Judite N Barbosa (Author) Other; Madureira, P (Author) Other; Mário Adolfo Barbosa (Author) FEUP; AP Águas (Author) ICBAS

Journal

Title: Biomaterials

Vol. 26 No. 5

Pages: 3021-3027

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-000-2Y2

DOI: 10.1016/j.biomaterials.2004.09.002

Abstract (EN): We have used self-assembled monolayers (SAMs) of alkanethiolates on gold to study the contribution of methyl terminal functional groups in implant-triggered inflammation. The CH3-coated biomaterials were inserted in an air-pouch cavity of the BALB/c mouse and the in situ inflammatory response was monitored 4, 24, 48 and 72 h later. Flow cytometry was applied to define surface expression of the adhesion receptor Mac-1 (CD11b/CD18), a marker of activated leukocytes, and also of CD3 and B220 antigens (T and B lymphocytes). The CH3-coated surfaces caused a significant enhancement in the number of Mac-1(+) cells in the implant. The only significant change in T and B lymphocytes was a transient increase in T cells detected 48 h after the implantation. Peak numbers of Mac-1(+) phagocytes were observed 24 h after implantation. We conclude that if CH3 is present at the surface of implants, this chemical group will trigger a significant enhancement of activated phagocytes involved in the inflammatory reaction, and this phenomenon may extend the local phlogistic event.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7