DIFFERENTIAL-EFFECTS OF 8 METAL-IONS ON LYMPHOCYTE DIFFERENTIATION ANTIGENS INVITRO

• Type: Article in International Scientific Journal
• Date: 1990

Authors

BRAVO, I (Author) Other; CARVALHO, GS (Author) Other; Mário Adolfo Barbosa (Author) FEUP; DESOUSA, M (Author) Other

Journal

Title: Journal of Biomedical Materials Research

Vol. 24

Pages: 1059-1068

ISSN: 0021-9304

Publisher: Heterocorporation

Indexing

ISI Web of Knowledge - 29 Citations

Scopus - 33 Citations

Other information

Authenticus ID: P-001-RKY

DOI: 10.1002/jbm.820240808

Abstract (EN): In vitro studies were conducted to determine the effects of metal ions known to be released from metallic implants in vivo on the expression of lymphocyte surface antigens. Normal human peripheral blood lymphocytes were exposed to various concentrations of metal ions (Fe3+, Ni2+, Co2+, Mo6+, V5+, Cr6+, Cr3+, and Ti3+) for 30 min at 37°C in a 5% CO2 atmosphere, and then analyzed for their ability to form rosettes with sheep red blood cells. Following this preliminary analysis, lymphocytes were exposed to the metal ions found to inhibit the E-rosette reaction (Fe3+, Ni2+, and Co2+) in order to determine which of the following surface antigens were affected: CD2, CD3, CD4, CD8, CD1, CD22, CD10, and HLA-DR. Our results showed that the in vitro treatment of lymphocytes with Fe3+ or Co2+ caused inhibition of CD2 only, whereas Ni2+ caused inhibition of both CD2 and CD3 antigens. These findings suggest that Fe3+, Co2+, and Ni2+ ions may interfere with T cell activation since both CD2 and CD3 are involved in that process.In vitro studies were conducted to determine the effects of metal ions known to be released from metallic implants in vivo on the expression of lymphocyte surface antigens. Results showed that the in vitro treatment of lymphocytes with Fe3+ or Co2+ caused inhibition of CD2 only, whereas Ni2+ caused inhibition of both CD2 and CD3 antigens. These findings suggest that Fe3+, Co2+, and Ni2+ ions may interfere with T cell activation since both CD2 and CD3 are involved in that process.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10