Influence of nitrogen/carbon ratio and complementary sugars on dextransucrase production by Leuconostoc mesenteroides NRRL B512(f).

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Lopretti, M (Author) Other; Martinez, E (Author) Other; Torres, L (Author) Other; Perdomo, R (Author) Other; Santos, M (Author) Other; Alírio Rodrigues (Author) FEUP

Journal

Title: Process Biochemistry

Vol. 34 No. 9

Pages: 879-884

ISSN: 1359-5113

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-001-38Z

DOI: 10.1016/s0032-9592(99)00020-5

Abstract (EN): The influence of the nitrogen/carbon ratio was studied during fermentation with Leuconostoc mesenteroides NRRL B512(f) using sucrose as substrate. The enzyme concentration was measured through activity tests and radiotracer tests with [C-14]phenylalanine. In batch fermentation a slowing down in the rate of enzyme synthesis was observed with a decrease in the nitrogen/carbon ratio. Addition of pulses of nitrogen when the nitrogen/carbon ratio decreased allowed a constant production rate of enzyme and lower fermentation time. The influence of complementary sugars was also addressed. Lactose inhibited enzyme production. When galactose was used the yield was the same as in the fermentation with sucrose alone, but with a different production rate. Maltose favoured the synthesis of dextransucrase.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6