Lactate dehydrogenase activity as an effect criterion in toxicity tests with Daphnia magna straus

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Diamantino, TC (Author) Other; Almeida, E (Author) Other; Soares, AMVM (Author) Other; Guilhermino, L. (Author) ICBAS

Journal

Title: Chemosphere

Vol. 45

Pages: 553-560

ISSN: 0045-6535

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 102 Citations

Scopus - 95 Citations

Other information

Authenticus ID: P-000-STR

DOI: 10.1016/s0045-6535(01)00029-7

Abstract (EN): Activity of lactate dehydrogenase (LDH) was used as an effect criterion in toxicity tests with Daphnia magna. In the first part of the work, the conditions for the use of LDH activity in toxicity tests with juveniles and adults of D. magna, were optimized. The influence of parameters such as the number of animals per sample, nutritional status, age and the presence of eggs in the brood chamber were investigated. In the second part of the study, both in vivo and in vitro tests based on the alteration of LDH activity of D. magna were developed and tested using zinc chloride as test substance. The results obtained indicate that LDH activity of D. magna may be used as an indicative parameter in aquatic toxicity tests. (C) 2001 Published by Elsevier Science Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8