Effects of carbofuran on the sea bass (Dicentrarchus labrax L.): Study of biomarkers and behaviour alterations

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Hernandez Moreno, D (Author) Other; Perez Lopez, M (Author) Other; Soler, F (Author) Other; Gravato, C (Author) Other; Guilhermino, L. (Author) ICBAS

Journal

Title: Ecotoxicology and Environmental Safety

Vol. 74

Pages: 1905-1912

ISSN: 0147-6513

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-002-M35

DOI: 10.1016/j.ecoenv.2011.07.016

Abstract (EN): The objective of this study was to investigate the acute effects of the pesticide carbofuran on the sea bass (Dicentrarchus labrax) using parameters at different levels of biological organisation (swimming behaviour and several biomarkers) and possible relationships between alterations found in different effect criteria. In a bioassay, sea bass juveniles were individually exposed to different doses of carbofuran (31, 63, 125 and 250 mu g/L) for 96 h. At the end of the bioassay, the swimming performance and 11 biomarkers were determined. Biomarkers were: hepatosomatic index (HSI), lipid peroxidation (LPO), reduced glutathione and the activities of the enzymes ethoxyresorufin O-deethylase (EROD), glutathione S-transferases, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, brain acetylcholinesterase (AChE) and muscle cholinesterases (ChE). After 96 h of exposure, carbofuran induced a decrease of the swimming velocity and inhibition of EROD activity at all concentrations tested, and inhibition of muscle ChE and brain AChE activities at 250 mu g/L. No relevant alterations in any of the other tested parameters were found. These results show that carbofuran induced adverse effects on fish by interfering with neurofunction, capability of detoxication and swimming velocity. In addition, positive and significant correlations between the swimming velocity and (i) brain AChE activity, (ii) muscle ChE activity and (iii) EROD activity suggest that the inhibition of these enzymes may somehow be related to the behavioural changes observed. Since these functions are determinant for the survival and performance of the fish in the wild, the findings of the present study suggest that adverse effects may occur in populations exposed to carbofuran if a sufficient number of animals is affected.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8