Polymer surface adsorption as a strategy to improve the biocompatibility of graphene nanoplatelets

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Artur M. Pinto (Author) FEUP; J. Agostinho Moreira (Author) Other; Fernão D. Magalhães (Author) FEUP; Inês C. Gonçalves (Author) Other

Journal

Title: Colloids and Surfaces B: Biointerfaces

Vol. 146

Pages: 818-824

ISSN: 0927-7765

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 38 Citations

Scopus - 39 Citations

INSPEC

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00K-VTR

DOI: 10.1016/j.colsurfb.2016.07.031

Abstract (EN): The biointeractions of graphene-based materials depend on their physico-chemical properties. These properties can be manipulated by polymer adsorption. Graphene nanoplatelets (GNP-C) were modified with PVA, HEC, PEG, PVP, chondroitin, glucosamine, and hyaluronic acid. These materials were characterized by SEM, DLS, XPS, Raman spectroscopy, and TGA. Surface adsorption was confirmed for all polymers. Biocompatibility evaluation showed that all of these materials induced low haemolysis (<1.7%) at concentrations up to 500 mu g mL(-1). GNP-C-PVA and GNP-C-HEC presented the lowest haemolysis percentages and were therefore more thoroughly studied. The morphology of HFF-1 cells was investigated by microscopy (optical, fluorescence, TEM) in order to evaluate interactions with GNP materials. Small GNP-C nanoplatelets were observed to enter cells independently of the surface treatment. For pristine GNP-C at a concentration of 50 mu g mL(-1), ROS production increased 4.4-fold. This effect is lower for GNP-C-PVA (3.3-fold) and higher for GNP-C-HEC (5.1-fold). Resazurin assays showed that GNP-C caused toxicity in HFF-1 cells at concentrations above 20 mu g mL(-1) at 24h, which decreased at 48 and 72 h. PVA surface adsorption rendered GNP-C non-toxic at concentrations up to 50 mu g mL(-1). LIVE/DEAD assays showed that at 20 and 50 mu g mL(-1) cell death is significantly lower for GNP-C-PVA compared to pristine GNP-C. Modification of nanoplatelets with HEC resulted in no benefit in terms of biocompatibility, whereas PVA considerably improved the biocompatibility.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7