Glycomic analysis of gastric carcinoma cells discloses glycans as modulators of RON receptor tyrosine kinase activation in cancer

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Mereiter, S (Author) Other; Magalhaes, A (Author) Other; Adamczyk, B (Author) Other; Jin, CS (Author) Other; Almeida, A (Author) Other; Drici, L (Author) Other; Ibanez Vea, M (Author) Other; Gomes, C (Author) Other; Ferreira, JA (Author) Other; Afonso, LP (Author) Other; Santos, LL (Author) Other; Larsen, MR (Author) Other; Kolarich, D (Author) Other; Karlsson, NG (Author) Other; Celso Reis (Author) ICBAS

Journal

Title: Biochimica et Biophysica Acta - General Subjects

Vol. 1860

Pages: 1795-1808

ISSN: 0304-4165

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 40 Citations

Scopus - 37 Citations

Other information

Authenticus ID: P-00K-NM2

DOI: 10.1016/j.bbagen.2015.12.016

Abstract (EN): Background: Terminal alpha 2-3 and alpha 2-6 sialylation of glycans precludes further chain elongation, leading to the biosynthesis of cancer relevant epitopes such as sialyl-Lewis X (SLe(X)). SLe(X) overexpression is associated with tumor aggressive phenotype and patients' poor prognosis. Methods: MKN45 gastric carcinoma cells transfected with the sialyltransferase ST3GAL4 were established as a model overexpressing sialylated terminal glycans. We have evaluated at the structural level the glycome and the sialoproteome of this gastric cancer cell line applying liquid chromatography and mass spectrometry. We further validated an identified target expression by proximity ligation assay in gastric tumors. Results: Our results showed that ST3GAL4 overexpression leads to several glycosylation alterations, including reduced O-glycan extension and decreased bisected and increased branched N-glycans. A shift from alpha 2-6 towards alpha 2-3 linked sialylated N-glycans was also observed. Sialoproteomic analysis further identified 47 proteins with significantly increased sialylated N-glycans. These included integrins, insulin receptor, carcinoembryonic antigens and RON receptor tyrosine kinase, which are proteins known to be key players in malignancy. Further analysis of RON confirmed its modification with SLe(X) and the concomitant activation. SLe(X) and RON co-expression was validated in gastric tumors. Conclusion: The overexpression of ST3GAL4 interferes with the overall glycophenotype of cancer cells affecting a multitude of key proteins involved in malignancy. Aberrant glycosylation of the RON receptor was shown as an alternative mechanism of oncogenic activation. General significance: This study provides novel targets and points to an integrative tumor glycomic/proteomic-profiling for gastric cancer patients' stratification. This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14