Role of E-cadherin N-glycosylation profile in a mammary tumor model

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Pinho, SS (Author) Other; Osorio, H (Author) Other; Nita Lazar, M (Author) Other; Gomes, J (Author) Other; Lopes, C (Author) Other; Gaertner, F (Author) Other; Celso Reis (Author) ICBAS

Journal

Title: Biochemical and Biophysical Research Communications

Vol. 379

Pages: 1091-1096

ISSN: 0006-291X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 58 Citations

Scopus - 68 Citations

Other information

Authenticus ID: P-003-N52

DOI: 10.1016/j.bbrc.2009.01.024

Abstract (EN): Modifications in cell surface glycosylation affecting cell adhesion are common characteristics of transformed cells. This study characterizes the N-glycosylation profile of E-cadherin in models of canine mammary gland adenoma and carcinoma evaluating the importance of these glycosylation modifications in the malignant phenotype. Our results show that the pattern of E-cadherin N-glycosylation in mammary carcinoma is characterized by highly branched N-glycans, increase in sialylation and an expression of few high mannose structures. Detailed mass spectrometry analysis demonstrated a new N-glycosylation site containing a potential complex type N-glycan in E-cadherin from a mammary carcinoma cell line. Our study demonstrates the importance of E-cadherin N-glycans in the process of tumor development and in the transformation to the malignant phenotype.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6