Stress test of a biological early warning system with zebrafish (Danio rerio)

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Amorim, J (Author) Other; Santos, MM (Author) FCUP; Vitor Vasconcelos (Author) FCUP; Luis Oliva Teles (Author) FCUP

Journal

Title: Ecotoxicology

Vol. 26

Pages: 13-21

ISSN: 0963-9292

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-00K-XHN

DOI: 10.1007/s10646-016-1736-5

Abstract (EN): The aim of this work was to develop a novel methodology to stress test the diagnostic capability of a video tracking system with zebrafish (Danio rerio), against two pre-established disturbances. Eight different treatments were tested varying the presence or absence of a toxicant (NaOCl) and two disturbances: the passing of a shadow (mimicking a predator) and entrapment of the fish. The concentration tested corresponded to a sublethal (1 % 24 h-LC50) and short term exposure (2 h). A total of 56 organisms were tested resulting in 112 diagnoses (before and after the contamination). A statistical model of diagnosis was developed using Self-organizing Map (SOM) and Correspondence Analysis (CA). Sensitivity, specificity, accuracy, false positive and false negative values were calculated to evaluate the diagnostic performance. The disturbances did not negatively affect the capability of the model. In the presence of at least one of these variables, the diagnostic performance was similar or even superior to the baseline results without disturbances. Furthermore, the system produced a large number of correct diagnoses, at an ecologically relevant concentration of exposure, in a non-invasive way.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9