The Effect of Ischemia and Reperfusion on Enteric Glial Cells and Contractile Activity in the Ileum

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Mendes, CE (Author) Other; Palombit, K (Author) Other; Vieira, C (Author) Other; Silva, I (Author) Other; Paulo Correia de Sá (Author) ICBAS; Castelucci, P (Author) Other

Journal

Title: Digestive Diseases and Sciences

Vol. 60

Pages: 2677-2689

ISSN: 0163-2116

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00G-3MG

DOI: 10.1007/s10620-015-3663-3

Abstract (EN): Background: We investigated the effects of ischemia followed by different periods of reperfusion (I/R) on immunoreactive S100ß-positive glial and Hu-immunoreactive neurons co-expressing the P2X2 receptor in the myenteric plexus of the rat ileum. Methods: The ileal artery was occluded for 35 min with an atraumatic vascular clamp. The animals were killed 24 h, 72 h, and 1 week after ischemia. Sham animals were not submitted to ileal artery occlusion. The relative density, size, and co-localization of P2X2 receptor-expressing cells in relation to S100ß-immunoreactive glial and Hu-immunoreactive neuronal cells were evaluated. Additionally, we analyzed the effects of I/R on gastrointestinal transit and ileum contractile activity. Results: The cellular density of P2X2 receptor and neuronal Hu immunoreactivity/cm2 decreased after I/R, whereas glial S100ß immunoreactivity/cm2 increased. No significant differences between sham and I/R groups were observed regarding the perikarya area of Hu-positive neurons. The area of S100ß-immunoreactive glial cells increased by 24.1 % 1 week after I/R compared with the 24 h group. Methylene blue progression along the small intestine decreased (P < 0.05) from 24.5 ± 2.3 % in the sham group to 17.2 ± 2.0 % 1 week post-ischemia. We noted a significant (P < 0.05) decrease in the maximal contraction amplitude triggered by electrical field stimulation in the presence of ATP in preparations submitted to 24 h of I/R. Conclusions: Changes in the P2X2 receptor density parallel myenteric neuronal loss following I/R of the rat ileum. This, together with the increase in the activated (oversized) glial cells, may contribute to decreased GI motility after I/R. © 2015, Springer Science+Business Media New York.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13