Osteopontin expression is correlated with differentiation and good prognosis in medullary thyroid carcinoma

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Ferreira, LB (Author) Other; Eloy, C (Author) Other; Pestana, A (Author) Other; Lyra, J (Author) Other; Moura, M (Author) Other; Prazeres, H (Author) Other; Tavares, C (Author) Other; Sobrinho Simoes, M (Author) FMUP; Gimba, E (Author) Other; Soares, P (Author) FMUP

Journal

Title: European Journal of Endocrinology

Vol. 174

Pages: 551-561

ISSN: 0804-4643

Publisher: BioScientifica Ltd.

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 20 Citations

Other information

Authenticus ID: P-00K-AZV

DOI: 10.1530/eje-15-0577

Abstract (EN): Background: Osteopontin (OPN) or secreted phosphoprotein 1 (SPP1) is a matricellular glycoprotein whose expression is elevated in various types of cancer and has been shown to be involved in tumourigenesis and metastasis in many malignancies, including follicular cell-derived thyroid carcinomas. Its role in C-cell-derived thyroid lesions and tumours remains to be established. Objective: The objective of this study is to clarify the role of OPN expression in the development of medullary thyroid carcinoma (MTC). Methods: OPN expression was analysed in a series of 116 MTCs by immunohistochemistry and by qPCR mRNA quantification of the 3 OPN isoforms (OPNa, OPNb and OPNc) in six cases from which fresh frozen tissue was available. Statistical tests were used to evaluate the relationship of OPN expression and the clinicopathological and molecular characteristics of patients and tumours. Results: OPN expression was detected in 91 of 116 (78.4%) of the MTC. We also observed high OPN expression in C-cell hyperplasia as well as in C-cells scattered in the thyroid parenchyma adjacent to the tumours. OPN expression was significantly associated with smaller tumour size, PTEN nuclear expression and RAS status, and suggestively associated with non-invasive tumours. OPNa isoform was expressed significantly at higher levels in tumours than in non-tumour samples. OPNb and OPNc presented similar levels of expression in all samples. Furthermore, OPNa isoform overexpression was significantly associated with reduced growth and viability in the MTC-derived cell line (TT). Conclusion: The expression of OPN in normal C-cells and C-cell hyperplasia suggests that OPN is a differentiation marker of C-cells, rather than a marker of biological aggressiveness in this setting. At variance with other cancers, OPN expression is associated with good prognostic features in MTC.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11