Lane background removal in thin-layer chromatography images using continuous wavelet

• Type: Article in International Conference Proceedings Book
• Date: 2012

Authors

Bruno M. Moreira (Author) FEUP; António V. Sousa (Author) Other; Ana Maria Mendonça (Author) FEUP; Aurélio Campilho (Author) FEUP

Conference proceedings National

Title: Atas do RecPad 2012

Pages: 1-2

Other information

Abstract (EN): This paper describes a new methodology to remove the background of the lanes in Thin-Layer Chromatography (TLC) images aiming at improving subsequent band detection. The storage of the biological samples to be analysed by TLC is usually done via plastic containers. Filter paper is an alternative that allows reduced costs and higher portability, but with consequences in the image analysis stage due to a lane background alteration. In order to overcome this problem, a negative control lane is generated in every chromatographic plate. After preprocessing and lane detection stages a one-dimensional intensity profile is used for integrating lane information and the background influence is removed with the help of the Continuous Wavelet Transform (CWT) decomposition. The proposed method was tested in 78 lane images, A band detection algorithm was applied on lane profiles, and a superior detection rate was achieved for the background removed lanes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 2