Effect of solvent system on extractability of lipidic components of Scenedesmus obliquus (M2-1) and Gloeothece sp. on antioxidant scavenging capacity thereof

• Type: Article in International Scientific Journal
• Date: 2015

Authors

F. Xavier Malcata (Author) FEUP; Helena M. Amaro (Author) Other; Fátima Fernandes (Author) Other; Patrícia Valentão (Author) Other; Paula B Andrade (Author) FFUP; Isabel Sousa Pinto (Author) FCUP; A. Catarina Guedes (Author) Other

Journal

Title: Marine Drugs

Serial No. 1660-3397 Vol. 13 No. 10

Pages: 6453-6471

ISSN: 1660-3397

Publisher: MDPI

Indexing

ISI Web of Knowledge - 54 Citations

ISI Web of Science

Scopus - 66 Citations

Scientific classification

CORDIS: Technological sciences

FOS: Engineering and technology

Associated Projects

UI0511 - Incentivo/EQB/UI0511/2014

Other information

Authenticus ID: P-00G-TBD

DOI: 10.3390/md13106453

Subject: carotenoid; PUFA; extract; microalga; cyanobacteria; ABTS+¿; DPPH¿; superoxide (O2¿¿) assay; nitric oxide (¿NO-) assay

Abstract (EN): Microalgae are well known for their biotechnological potential, namely with regard to bioactive lipidic components¿especially carotenoids and polyunsaturated fatty acids (PUFA), well-known for therapeutic applications based on their antioxidant capacity. The aim of this work was to evaluate the influence of four distinct food-grade solvents upon extractability of specific lipidic components, and on the antioxidant capacity exhibited against both synthetic (2,2-diphenyl-1-picrylhydrazyl (DPPH¿) and 2,2¿-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+¿)) and biological reactive species (O2¿- and ¿NO-). A eukaryotic microalga (Scenedesmus obliquus (M2-1)) and a prokaryotic one (Gloeothece sp.) were used as case studies. Concerning total antioxidant capacity, the hexane:isopropanol (3:2) and acetone extracts of Sc. obliquus (M2-1) were the most effective against DPPH¿ and ABTS+¿, respectively. Gloeothece sp. ethanol extracts were the most interesting scavengers of O2¿-, probably due the high content of linolenic acid. On the other hand, acetone and hexane:isopropanol (3:2) extracts were the most interesting ones in ¿NO- assay. Acetone extract exhibited the best results for the ABTS assay, likely associated to its content of carotenoids, in both microalgae. Otherwise, ethanol stood out in PUFA extraction. Therefore, profiles of lipidic components extracted are critical for evaluating the antioxidant performance¿which appears to hinge, in particular, on the balance between carotenoids and PUFAs.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 19