The prevalence of PIK3CA mutations in gastric and colon cancer

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Velho, S (Author) Other; Oliveira, C (Author) Other; Ferreira, A (Author) Other; Ferreira, AC (Author) Other; Suriano, G (Author) Other; Schwartz, S (Author) Other; Duval, A (Author) Other; Carneiro F (Author) FMUP; Machado JC (Author) FMUP; Hamelin, R (Author) Other; Seruca, R (Author) FMUP

Journal

Title: European Journal of Cancer

Vol. 41

Pages: 1649-1654

ISSN: 0959-8049

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 295 Citations

Scopus - 311 Citations

Other information

Authenticus ID: P-000-2H0

DOI: 10.1016/j.ejca.2005.04.022

Abstract (EN): A wide variety of tumours show PIK3CA mutations leading to increased phosphatidylinositol-3 kinase (PI3K) activity. We have determined the frequency of PIK3CA mutations in exons 9 and 20 that has previously been reported as mutational hotspot regions in distinct tumour models. One hundred and fifty gastrointestinal carcinomas (47 gastric and 103 colorectal) that were characterised for MSI status (76 MSI and 74 MSS) by PCR-SSCP sequencing were evaluated. We also analysed the association between PIK3 CA mutations and KRAS or BRAF mutations. PIK3CA mutations in exons 9 and 20 were present in 13.6% and 10.6% of colorectal and gastric carcinomas, respectively. No differences in frequency and type of PIK3CA mutations were found between MSI and MSS colorectal carcinomas. All gastric carcinomas with PIK3CA mutations were MSI. The number of cases harbouring concomitant PIK3 CA and KRAS or BRAF mutations was higher in colorectal than in gastric carcinomas (P = 0.016). In colorectal carcinoma, PIK3CA mutations occur preferentially together with activating KRAS-BPAF mutations (MSI and MSS) while in gastric carcinomas PIK3CA mutations tend to occur as isolated events (MSI).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6