Influence of flow velocity on the characteristics of pseudomonas fluorescens biofilms

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Paula A. Araújo (Author) FEUP; Joana Malheiro (Author) FEUP; Idalina Machado (Author) FEUP; Filipe Mergulhão (Author) FEUP; Luís Melo (Author) FEUP; Manuel Simões (Author) FEUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: Journal of Environmental Engineering (United States)

Vol. 142 No. 7

Pages: 04016031-1-04016031-8

ISSN: 0733-9372

Indexing

ISI Web of Knowledge - 14 Citations

ISI Web of Science

Scopus - 23 Citations

INSPEC

Associated Projects

LEPAE/CEFT - RL2 - (Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

NOVOMAR - Consolidação do Centro Multipolar de Valorização de Recursos Marinhos visando novos usos do mar

Other information

Authenticus ID: P-00K-JKW

DOI: 10.1061/(asce)ee.1943-7870.0001068

Abstract (EN): The characteristics of Pseudomonas fluorescens biofilms formed under three different linear flow velocities (u=0.1, 0.4, and 0.8 m/s; Reynolds numbers of 1,000, 4,000, and 8,000, respectively) were studied providing an extension to the studies of earlier researchers. A flow cell reactor system was used to form biofilms, and they were characterized in terms of thickness, morphological structure, mass, cell density, outer membrane protein expression, and matrix and total protein and polysaccharide content. The external mass transfer coefficients were also assessed. The biofilms developed at u=0.4 and 0.8 m/s had similar characteristics but were significantly different from those developed at 0.1 m/s. High flow velocities formed thinner biofilms with higher cell densities and contents of matrix/extracellular proteins and polysaccharides. The increase of flow velocity from 0.4 to 0.8 m/s caused a higher production of matrix proteins and polysaccharides. The external mass transfer coefficients suggest mass transfer limitations for the lowest velocity. Scanning electron microscopy images show cell-surface and cell-cell attachment structures appearing more frequently in biofilms formed at the two higher velocities. No major differences were found in the outer membrane protein expression of biofilm cells formed under the selected linear flow velocities. The overall results show the effect of the hydrodynamic conditions under which biofilms were formed on selected macromolecular characteristics, demonstrating that higher flow velocities can originate more complex and denser biofilms. © 2016 American Society of Civil Engineers.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8