Lewis enzyme (alpha 1-3/4 fucosyltransferase) polymorphisms do not explain the Lewis phenotype in the gastric mucosa of a Portuguese population

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Serpa, J (Author) Other; Raquel Almeida (Author) Other; Oliveira, C (Author) Other; Santos-Silva F (Author) Other; Silva, E (Author) Other; Celso Reis (Author) Other; Le Pendu, J (Author) Other; José Oliveira (Author) FMUP; Ribeiro, LMC (Author) Other; David L (Author) FMUP

Journal

Title: Journal of Human Genetics

Vol. 48

Pages: 183-189

ISSN: 1434-5161

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-000-K0H

DOI: 10.1007/s10038-003-0007-5

Abstract (EN): The human alpha-1,3/4 fucosyltransferase III (FucT III) catalyses the synthesis of Lewis antigens including Le(b) antigen which is a ligand for Helicobacter pylori adhesion. Several polymorphisms have been described in the FUT3 gene affecting both the transmembrane and catalytic domains, some of which affect the enzyme activity. The aim of the present work was to study the Lewis gene polymorphisms in a Caucasian Portuguese population, with a high rate of H. pylori infection, and to evaluate the implications of mutant enzymes in Le(b) expression in the gastric mucosa. We studied 460 asymptornatic or dyspeptic individuals from northern Portugal. Screening for Lewis gene polymorphisms was performed by SSCP and direct sequencing. Lewis phenotype in gastric mucosa was determined by immunohistochemistry. In 47 individuals with a Lewis negative blood group, we found FUT3 gene polymorphisms that were previously described in other populations: 59T > G, 202T > C, 314C > T, 508G > A and 1067T > A. Among the 47 Lewis negative individuals in blood, only nine were also negative in gastric mucosa, suggesting the existence of another alpha 1-4 fucosyltransferase that is responsible for Le(a) and Le(b) synthesis in gastric mucosa.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7