Heterogeneous and homogeneous immunoassays for drug analysis

• Type: Another Publication in an International Scientific Journal
• Date: 2014

Authors

Ricardo Jorge Dinis Oliveira (Author) FMUP

Journal

Title: Bioanalysis

Vol. 6

Pages: 2877-2896

ISSN: 1757-6180

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 27 Citations

Other information

Authenticus ID: P-00A-1EQ

DOI: 10.4155/bio.14.208

Abstract (EN): Immunoassays are very useful techniques to perform screening and semi-quantitative analysis of hundreds of different xenobiotics. Small sample volumes are required and pretreatment is usually unnecessary (e.g., homogeneous immunoassays). Fully automated and high-throughput systems are available, which help physicians to take timely decisions. However, immunoassays do suffer from interference from both endogenous and exogenous factors that limit their application in quantitative analysis. These assays use different labels (e.g., colorimetric, fluorescent, chemiluminescent or electrochemiluminescent) and different methods for generating and measuring signals, but the basic principles are usually similar. This review outlines the practical aspects of immunoassays in bioanalysis and describes their application in clinical chemistry for xenobiotic analysis, namely medicines and drugs of abuse.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 20