Effect of a Purification Step and the Type of Internal Standard Used on Fatty Acid Determination of Grass and Maize Silages

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Alves, SP (Author) Other; A. Cabrita (Author) FCUP; Antonio J M Fonseca (Author) ICBAS; Bessa, RJB (Author) Other

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 57 No. 19

Pages: 10793-10797

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 7 Citations

Scientific classification

CORDIS: Natural sciences > Agrarian Sciences > Zootechnics

FOS: Agrarian Sciences > Animal and Dairy science

Other information

Authenticus ID: P-003-E9Y

DOI: 10.1021/jf901769w

Abstract (EN): The fatty acid (FA) analysis of grass and maize silages was studied by application of a direct transesterification method (DT) followed by purification by solid-phase extraction (SPE). The choice of the internal standard (IS) for quantification of FA by gas-liquid chromatography (GLC) was also studied. The acidic DT method applied to grass silage samples produced a high amount of non-fatty acid methyl ester compounds (non-FAME) compared with those formed in maize silages. The application of the SPE cleanup step reduced significantly the amount of non-FAME compounds in both samples. Five FAs were tested as IS; among them, 3 were naturally present in all silages, however their use as IS did not affect quantification of total FA composition. Nevertheless, some minor FAs present in silages were significantly affected by the IS used. Additionally, application of corrections to the GLC peak areas did not significantly influence quantification of total FA composition of silages.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5