Improved method for fatty acid analysis in herbage based on direct transesterification followed by solid-phase extraction

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Alves, SP (Author) Other; A. Cabrita (Author) FCUP; Antonio J M Fonseca (Author) ICBAS; Bessa, RJB (Author) Other

Journal

Title: Journal of Chromatography A

Vol. 1209

Pages: 212-219

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 39 Citations

Scopus - 41 Citations

Scientific classification

CORDIS: Natural sciences > Agrarian Sciences > Zootechnics

FOS: Agrarian Sciences > Animal and Dairy science

Other information

Authenticus ID: P-003-VE9

DOI: 10.1016/j.chroma.2008.08.114

Abstract (EN): Direct transesterification (DT) and solvent extraction with acid or basic derivatization procedure for fatty acid (FA) analysis in herbage were compared. The highest total FA, alpha-linolenic and linoleic acid contents were obtained with DT. However, DT also produced the highest amount of interfering compounds, identified as phytadienes and sugar derivative products, which may co-elute with FA. An additional step based on solid-phase extraction to produce clean samples was proposed. This procedure is simple and gives good recoveries for the FA fortified samples. Additionally, structural characterization of 16:1 trans-3 was conducted by covalent adduct chemical ionization tandem mass spectrometry.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8