Isolation and characterization of two new methanesulfonic acid-degrading bacterial isolates from a Portuguese soil sample

• Type: Article in International Scientific Journal
• Date: 2000

Authors

De Marco, P (Author) Other; Murrell, JC (Author) Other; Adriano Bordalo (Author) ICBAS; Pedro Moradas Ferreira (Author) ICBAS

Journal

Title: Archives of Microbiology

Vol. 173

Pages: 146-153

ISSN: 0302-8933

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-001-0VK

DOI: 10.1007/s002039900124

Abstract (EN): Two novel bacterial strains that can utilize methanesulfonic acid as a source of carbon and energy were isolated from a soil sample collected in northern Portugal. Morphological, physiological, biochemical and molecular biological characterization of the two isolates indicate that strain P1 is a pink-pigmented facultative methylotroph belonging to the genus Methylobacterium, while strain P2 is a restricted methylotroph belonging to the genus Hyphomicrobium. Both strains are strictly aerobic, degrade methanesulfonate, and release small quantities of sulfite into the medium. Growth on methanesulfonate induces a specific polypeptide profile in each strain. This, together with the positive hybridization to a DNA probe that carries the msm genes of Methylosulfonomonas methylovora strain M2, strongly endorses the contention that a methanesulfonic acid monooxygenase related to that found in the previously known methanesulfonate-utilizing bacteria is present in strains P1 and P2. The isolation of bacteria containing conserved msm genes from diverse environments and geographical locations supports the hypothesis that a common enzyme may be globally responsible for the oxidation of methanesulfonate by natural methylotrophic communities.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8