The Drosophila RAD21 cohesin persists at the centromere region in mitosis

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Warren, WD (Author) Other; Steffensen, S (Author) Other; Lin, E (Author) Other; Coelho, P (Author) Other; Loupart, ML (Author) Other; Cobbe, N (Author) Other; Lee, JY (Author) Other; McKay, MJ (Author) Other; Orr Weaver, T (Author) Other; Heck, MMS (Author) Other; Sunkel, CE (Author) ICBAS

Journal

Title: Current Biology

Vol. 10

Pages: 1463-1466

ISSN: 0960-9822

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 104 Citations

Scopus - 105 Citations

Other information

Authenticus ID: P-000-YG1

DOI: 10.1016/s0960-9822(00)00806-x

Abstract (EN): 'Cohesin' is a highly conserved multiprotein complex thought to be the primary effector of sister-chromatid cohesion in all eukaryotes. Cohesin complexes in budding yeast hold sister chromatids together from S phase until anaphase, but in metazoans, cohesin proteins dissociate from chromosomes and redistribute into the whole cell volume during prophase, well before sister chromatids separate (reviewed in [1,2]). Here we address this apparent anomaly by investigating the cell-cycle dynamics of DRAD21, the Drosophila orthologue of the Xenopus XRAD21 and Saccharomyces cerevisiae Scc1p/Mcd1p cohesins [3]. Analysis of DRAD21 in S2 Drosophila tissue culture cells and live embryos expressing a DRAD21-green fluorescent protein (GFP) fusion revealed the presence of four distinct subcellular pools of DRAD21: a cytoplasmic pool; a chromosome-associated pool which dissociates from chromatin as chromosomes condense in prophase; a short-lived centrosome-associated pool present during meta phase-ana phase; and a centromere-proximal pool which remains bound to condensed chromosomes, is found along the junction of sister chromatids between kinetochores, and persists until the meta phase-anaphase transition. We conclude that in Drosophila, and possibly all metazoans, a minor pool of cohesin remains bound to centromere-proximal chromatin after prophase and maintains sister-chromatid cohesion until the meta ph ase-anaphase transition.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4