Drosophila CENP-C is essential for centromere identity

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Orr, B (Author) Other; Sunkel, CE (Author) ICBAS

Journal

Title: Chromosoma

Vol. 120

Pages: 83-96

ISSN: 0009-5915

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 21 Citations

Other information

Authenticus ID: P-002-VEK

DOI: 10.1007/s00412-010-0293-6

Abstract (EN): Centromeres are specialized chromosomal domains that direct mitotic kinetochore assembly and are defined by the presence of CENP-A (CID in Drosophila) and CENP-C. While the role of CENP-A appears to be highly conserved, functional studies in different organisms suggest that the precise role of CENP-C in kinetochore assembly is still under debate. Previous studies in vertebrate cells have shown that CENP-C inactivation causes mitotic delay, chromosome missegregation, and apoptosis; however, in Drosophila, the role of CENP-C is not well-defined. We have used RNA interference depletion in S2 cells to address this question and we find that depletion of CENP-C causes a kinetochore null phenotype, and consequently, the spindle checkpoint, kinetochore-microtubule interactions, and spindle size are severely misregulated. Importantly, we show that CENP-C is required for centromere identity as CID, MEI-S332, and chromosomal passenger proteins fail to localize in CENP-C depleted cells, suggesting a tight communication between the inner kinetochore proteins and centromeres. We suggest that CENP-C might fulfill the structural roles of the human centromere-associated proteins not identified in Drosophila.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14