Strategies for outcrossing and genetic manipulation of Drosophila compound autosome stocks.

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Martins, T (Author) Other; Kotadia, S (Author) Other; Malmanche, N (Author) Other; Sunkel, CE (Author) ICBAS; Sullivan, W (Author) Other

Journal

Title: G3: Genes, Genomes, Genetics

Vol. 3

Pages: 1-4

ISSN: 2160-1836

Publisher: Genetics Society of America

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00G-GTA

DOI: 10.1534/g3.112.004481

Abstract (EN): Among all organisms, Drosophila melanogaster has the most extensive well-characterized collection of large-scale chromosome rearrangements. Compound chromosomes, rearrangements in which homologous chromosome arms share a centromere, have proven especially useful in genetic-based surveys of the entire genome. However, their potential has not been fully realized because compound autosome stocks are refractile to standard genetic manipulations: if outcrossed, they yield inviable aneuploid progeny. Here we describe two strategies, cold-shock and use of the bubR1 mutant alleles, to produce nullo gametes through nondisjunction. These gametes are complementary to the compound chromosome-bearing gametes and thus produce viable progeny. Using these techniques, we created a compound chromosome two C(2)EN stock bearing a red fluorescent protein-histone transgene, facilitating live analysis of these unusually long chromosomes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4