Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Ramos, AA (Author) Other; Prata Sena, M (Author) Other; Castro Carvalho, B (Author) Other; Dethoup, T (Author) Other; Buttachon, S (Author) Other; Kijjoa, A (Author) Other; rocha, e (Author) ICBAS

Journal

Title: Asian Pacific Journal of Tropical Medicine

Vol. 8

Pages: 782-790

ISSN: 1995-7645

Publisher: Wolters Kluwer Health

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-00G-TM6

DOI: 10.1016/j.apjtm.2015.09.005

Abstract (EN): Objective: To evaluate the in vitro anticancer activity of crude ethyl acetate extracts of the culture of four marine-derived fungi Aspergillus similanensis KUFA 0013 (El), Neosartorya paulistensis KUFC 7897 (E2), Neosartorya siamensis KUFA 0017 (E4) and Talaromyces trachyspermns KUFC 0021 (E3) on a panel of seven human cancer cell lines. Methods: Effects on cell proliferation, induction of DNA damage and cell death were assessed by MTT and clonogenic assays, cornet assay and nuclear condensation assay, respectively. Results: The proliferation of HepC2. HCT116 and A375 cells decreased after incubation with the extracts E2 and E4. The anti-proliferative effect was confirmed by morphologic alterations and by clonogenic assay. Both extracts also induced cell death in HepG2 and HCT116 cells. Doxorubicin was used as a positive control and showed in vitro anticancer activity. Conclusions: This study demonstrated, for the first time, that extracts of Neosartorya panlistensis and Neosartorya siamensis have selective anti-proliferative and cell death activities in HepG2. HCT16 and A375 cells. The bioactivity of these extracts suggests a potential for biotechnological applications and substantiates that both should be further considered for the elucidation of the molecular targets and signal transduction pathways involved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9