Successful pregnancy after spermatid injection

• Type: Article in International Scientific Journal
• Date: 1998

Authors

Bernabeu, R (Author) Other; Cremades, N (Author) Other; Takahashi, K (Author) Other; sousa, m (Author) ICBAS

Journal

Title: Human Reproduction

Vol. 13

Pages: 1898-1900

ISSN: 0268-1161

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 41 Citations

Other information

Authenticus ID: P-001-7JA

DOI: 10.1093/humrep/13.7.1898

Abstract (EN): We present nine cases of spermatid intracytoplasmic injection for the treatment of non-obstructive azoospermia, In eight cases, no elongated spermatids or spermatozoa mere found in previous spermiograms or testicular biopsies, In these patients, treatment was performed using ejaculated (n = 6) and testicular(n = 2) retrieved round spermatids (Sa type). In cases where ejaculated round spermatids were used, they mere isolated on the day before oocyte retrieval and left in culture for 24 h before intracytoplasmic sperm injection (ICSI), No pregnancy was obtained in either group, although culturing seemed to increase the fertilization rate. In one other case, elongated spermatids were observed in the previous spermiogram and thus a normal ICSI procedure was scheduled. However, on the day of oocyte retrieval, no spermatids could be recovered from fresh sequential ejaculates, and a testicular open biopsy was then performed. Both round and elongated spermatids were found in the testicular tissue, but only the more mature germinal cells (Sb2) were injected. From this case, a normal pregnancy was obtained which resulted in the birth by Caesarean section at 37 weeks of gestation of a normal healthy baby girl, weighing 2700 g.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3