Activation of mouse oocytes requires multiple sperm factors but not sperm PLC gamma 1

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Heyers, S (Author) Other; sousa, m (Author) ICBAS; Cangir, O (Author) Other; Schmoll, F (Author) Other; Schellander, K (Author) Other; van der Ven, H (Author) Other; Montag, M (Author) Other

Journal

Title: Molecular and Cellular Endocrinology

Vol. 166

Pages: 51-57

ISSN: 0303-7207

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-000-Z8B

DOI: 10.1016/s0303-7207(00)00297-5

Abstract (EN): A sperm cytosolic factor is responsible for oocyte activation at fertilization in mammals. The molecular identity of this factor is not yet known, although a sperm phospholipase C gamma (PLC gamma) is a potential candidate. In this study, cation-exchange chromatography with a Heparin column was used for the fractionation of porcine sperm cytosolic extracts. Oocyte activation potential of the resulting fractions was tested and active fractions were subjected to Western blot analysis using antibodies specific to PLC gamma 1. PLC gamma 1 was detected in fractions other than those supporting oocyte activation (Ca2+-release and pronuclear formation). The active Heparin fraction was then purified on a Mono Q anion-exchange column. One of the resulting fractions still contained Ca2+-releasing activity, bur pronuclear formation did not occur. We conclude that sperm PLC gamma 1 is not involved in oocyte activation and that Ca2+-release and pronuclear formation requires multiple factors from sperm cytosol.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7