Effect of insulin deprivation on metabolism and metabolism-associated gene transcript levels of in vitro cultured human Sertoli cells

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Oliveira, PF (Author) Other; Alves, MG (Author) Other; Rato, L (Author) Other; Laurentino, S (Author) Other; Silva, J (Author) Other; Sa, R (Author) ICBAS; barros, a (Author) FMUP; sousa, m (Author) ICBAS; Carvalho, RA (Author) Other; cavaco, je (Author) Other; Socorro, S (Author) Other

Journal

Title: Biochimica et Biophysica Acta - General Subjects

Vol. 1820

Pages: 84-89

ISSN: 0304-4165

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 105 Citations

Scopus - 116 Citations

Other information

Authenticus ID: P-002-DER

DOI: 10.1016/j.bbagen.2011.11.006

Abstract (EN): Background: Sertoli cells metabolize glucose producing lactate for developing germ cells. As insulin regulates glucose uptake and its disturbance/insensitivity is associated with diabetes mellitus, we aimed to determine the effect of insulin deprivation in human Sertoli cell (hSC) metabolism and metabolism-associated gene expression. Methods: hSC-enriched primary cultures were maintained in the absence/presence of insulin and metabolite variations were determined by H-1-NMR. mRNA expression levels of glucose transporters (GLUT1, GLUT3), lactate dehydrogenase (LDHA) and monocarboxylate transporter (MCT4) were determined by RT-PCR. Results: Insulin deprivation resulted in decreased lactate production and in decrease of glucose consumption that was completely reverted after 6 h. Cells of both groups consumed similar amounts of glucose. In insulin-deprived cells, transcript levels of genes associated to lactate metabolism (LDHA and MCT4) were decreased. Transcript levels of genes involved in glucose uptake exhibited a divergent variation: GLUT3 levels were decreased while GLUT1 levels increased. Insulin-deprived hSCs presented: 1) altered glucose consumption and lactate secretion; 2) altered expression of metabolism-associated genes involved in lactate production and export; 3) an adaptation of glucose uptake by modulating the expression of GLUT1 and GLUT3. General significance: This is the first report regarding the effect of insulin-deprivation on hSC metabolism.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6