A new methodology for the improvement of diagnostic immunohistochemistry in canine veterinary pathology: automated system using human monoclonal and polyclonal antibodies

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Cassali, GD (Author) Other; Silva, P (Author) Other; Rema, A (Author) Other; Fátima Gärtner (Author) ICBAS; Gobbi, H (Author) Other; Tafuri, WL (Author) Other; Fernando Schmitt (Author) FMUP

Journal

Title: Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

Vol. 53

Pages: 326-331

ISSN: 0102-0935

Publisher: Universidade Federal de Minas Gerais

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-000-V8X

DOI: 10.1590/s0102-09352001000300010

Abstract (EN): The authors describe their experience with an automated immunohistochemical system applied to canine tissue samples. Twenty human cellular markers specific monoclonal and polyclonal antibodies and two different antigen retrieval methods were used in normal and neoplastic breast tissue, as well as skin samples obtained from female dogs of pure and mixed breeds. The antibodies tested were the most frequently used in human and veterinary medicine studies, employed with diagnostic purposes in breast pathology, as well as in cancer research. Most of them may be used to study other normal and abnormal tissues and included cytokeratins, progesterone receptor, c-erbB2, p53, MIB-1, PCNA, EMA, vimentin, desmin, alpha -actin, S-100, pan-cadherin, and E-cadherin. The results demonstrated that using an automated staining system it is possible to use different human markers in veterinary pathology. The advantages of automated immunohistochemistry are improved quality, reproducibility, speed, and standardisation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6