Somatic embryogenesis, organogenesis and callus growth kinetics of flax

• Type: Article in International Scientific Journal
• Date: 1997

Authors

daCunha, ACG (Author) Other; Ferreira, MF (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 47

Pages: 1-8

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 3 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-CV4

Abstract (EN): The effects of plant growth regulators (PGR) on calli induction, morphogenesis and somatic embryogenesis of flax were studied. The organogenic and callus formation capacity were assessed for different types of source explants. Root and shoot explants were equally good material for calli production but the former produced calli without shoot regeneration capacity. Under the experimental conditions tested, 2,4-dichlorophenoxyacetic acid (2,4-D) + zeatin was the most efficient PGR combination on calli induction and biomass production. The calli were green but with no rhizogenic capacity. In contrast, and at similar concentrations, indole-3-butyric acid (IBA) + kinetin induced white or pale green friable calli with a good root regeneration capacity (60%). A factorial experiment with different combinations of 2,4-D + zeatin + gibberellic acid (GA(3)) levels revealed that the direction of explant differentiation was determined by specific PGR interactions and concentrations. The results from these experiments revealed that the morphogenetic pathway (shoot versus root differentiation) can be manipulated on flax explants by raising the 2,4-D level from 0.05 to 3.2 mg l(-1) in the induction medium. The induction and development of somatic embryos from flax explants was possible in a range of 2,4-D + zeatin concentrations surrounding 0.4 mg l(-1) 2,4-D and 1.6 mg l(-1) zeatin, the most efficient growth regulator combination.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8