Dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Coutinho, F (Author) Other; Castro, C (Author) Other; Rufino Palomares, E (Author) Other; Ordonez Grande, B (Author) Other; Gallardo, MA (Author) Other; Oliva Teles, A (Author) FCUP; Peres, H (Author) FCUP

Journal

Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Vol. 191

Pages: 9-17

ISSN: 1095-6433

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 36 Citations

Scopus - 39 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-00G-PGY

DOI: 10.1016/j.cbpa.2015.09.012

Abstract (EN): A study was undertaken to evaluate dietary glutamine supplementation effects on gilthead sea bream performance, intestinal nutrient absorption capacity, hepatic and intestinal glutamine metabolism and oxidative status. For that purpose gilthead sea bream juveniles (mean weight 13.0 g) were fed four isolipidic (18% lipid) and isonitrogenous (43% protein) diets supplemented with 0, 0.5, 1 and 2% glutamine for 6 weeks. Fish performance, body composition and intestinal nutrient absorption capacity were not affected by dietary glutamine levels. Hepatic and intestinal glutaminase (GINase), glutamine synthetase (GSase), alanine aminotransferase, aspartate aminotransferase and glutamate dehydrogenase activities were also unaffected by dietary glutamine supplementation. In the intestine GINase activity was higher and GSase/GINase ratio was two-fold lower than in the liver, suggesting a higher use of glutamine for energy production by the intestine than by the liver. The liver showed higher catalase and glucose-6-phosphate dehydrogenase activities, while the intestine presented higher glutathione peroxidase and glutathione reductase activities and oxidised glutathione content, which seems to reveal a higher glutathione dependency of the intestinal antioxidant response. Total and reduced glutathione contents in liver and intestine and superoxide dismutase activity in the intestine were enhanced by dietary glutamine, though lipid peroxidation values were not affected. Overall, differences between liver and intestine glutamine metabolism and antioxidant response were identified and the potential of dietary glutamine supplementation to gilthead sea bream's antioxidant response was elucidated.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9