A LONG PERIOD GRATING-BASED PLATFORM FOR THE DETECTION OF E. COLI PROTEINS

• Type: Article in International Conference Proceedings Book
• Date: 2013

Authors

Queiros, RB (Author) Other; Gouveia, C (Author) Other; Fernandes, JRA (Author) Other; Marques, PVS (Author) FCUP; Noronha, JP (Author) Other; Sales, MGF (Author) Other; Jorge, PAS (Author) FCUP

Conference proceedings International

Title: 8TH IBEROAMERICAN OPTICS MEETING AND 11TH LATIN AMERICAN MEETING ON OPTICS, LASERS, AND APPLICATIONS

8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications

Porto, PORTUGAL, JUL 22-26, 2013

Indexing

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Scientific classification

FOS: Natural sciences > Physical sciences

Other information

Authenticus ID: P-006-K51

DOI: 10.1117/12.2025811

Abstract (EN): A Long Period Grating (LPG)-based platform for the detection of E. coli outer membranes proteins (EcOMPs) is presented. The sensing probe is achieved by the functionalization of a LPG inscribed in a single mode fiber (SMF28) with a DNA-aptamer resulting in a label-free configuration capable of specific recognize EcOMPs in waters. Measurement takes place by tracking the variations induced in the resonance wavelength by the refractive index changes at the fiber surface (approximate to 100 nm/RIU). The sensing head was characterized and tested against EcOMPs and applied to spiked environmental water samples. The sensor displayed logarithmic responses in the range of 0.1 nM to 10 nM EcOMPs and is regenerated (under low pH conditions) and the deviation of the subsequent detection was less than 0.1%.

Language: English

Type (Professor's evaluation): Scientific