Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Sonia Soares (Author) Other; Joana S Amaral (Author) Other; Beatriz B P P Oliveira (Author) FFUP; Isabel Mafra (Author) Other

Journal

Title: Meat Science

Vol. 98

Pages: 41-46

ISSN: 0309-1740

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 20 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-009-HKN

DOI: 10.1016/j.meatsci.2014.04.002

Abstract (EN): In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%, being successfully in-house validated. Its application was effective in the analysis of several meat products, indicating 2% of non-compliance with the food allergen labelling legislation, and some inconsistencies when comparing the declared with estimated amounts of soybean. This work highlights the importance of efficient tools to assess labelling statements of meat products, avoiding fraudulent practices.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6