Bioactive compounds and scavenging capacity of extracts from different parts of Vismia cauliflora against reactive oxygen and nitrogen species

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Alessandra Braga Ribeiro (Author) Other; Alessandra Berto (Author) Other; Renan Campos Chiste (Author) Other; Marisa Freitas (Author) Other; Jesui V Visentainer (Author) Other; Eduarda Fernandes (Author) FFUP

Journal

Title: Pharmaceutical Biology

Vol. 53

Pages: 1267-1276

ISSN: 1388-0209

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 13 Citations

Scientific classification

FOS: Engineering and technology > Medical engineering

Other information

Authenticus ID: P-00G-S28

DOI: 10.3109/13880209.2014.974063

Abstract (EN): Context: Vismia cauliflora A.C.Sm. [ Hypericaceae (Clusiaceae)] is a plant from Amazonian forest. It is used by Amerindians to treat dermatosis and inflammatory processes in the skin and has been considered an interesting source of bioactive compounds. Objective: We evaluated the scavenging capacity of extracts from V. cauliflora (leaf, branch, stem bark, flower, and whole fruit) against reactive oxygen (ROS) and nitrogen species (RNS), namely, superoxide radical (O-2(center dot)), hydrogen peroxide (H2O2), hypochlorous acid (HOCl), singlet oxygen (O-1(2)), nitric oxide ((NO)-N-center dot), and peroxynitrite (ONOO-). In addition, for the first time, the profile of phenolic compounds and carotenoids was determined. Materials and methods: The scavenging capacities of each extract were determined using specific probes (fluorescent, colorimetric, and chemiluminescent) to detect different reactive species (O-1(2), HOCl, H2O2, O-2(center dot), (NO)-N-center dot and ONOO-). The identification and the quantification of phenolic compounds and carotenoids were carried out by HPLC-DAD-ESI-MS/MS and HPLC-DAD, respectively. Results: (-)-Epicatechin and proanthocyanidin dimers and trimer were the major phenolic compounds tentatively identified in leaf, branch, stem bark, and flower extracts, while dihydroxybenzoic acids were the major compounds in whole fruit extracts. All-trans-zeinoxanthin and all-trans-beta-carotene were the major carotenoids tentatively identified in leaf extracts. All extracts of V. cauliflora showed high efficiency against all tested ROS and RNS, although flower and stem bark extracts exhibited the most remarkable scavenging capacity, especially for (NO)-N-center dot and ONOO-. Discussion and conclusion: Vismia cauliflora has great potential to be used in the development of phytopharmaceutical products due to its characteristic of being a promising source of bioactive compounds with high antioxidant properties.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10