A multiplex PCR assay for identification of the red fox (Vulpes vulpes) using the mitochondrial ribosomal RNA genes

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Joana Goncalves (Author) Other; Clive A Marks (Author) Other; David Obendorf (Author) Other; Antonio Amorim (Author) FCUP; Filipe Pereira (Author) Other

Journal

Title: Conservation Genetics Resources

Vol. 7

Pages: 45-48

ISSN: 1877-7252

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-00A-64W

DOI: 10.1007/s12686-014-0343-0

Abstract (EN): The European red fox (Vulpes vulpes) is a highly adaptable predator indigenous to the northern hemisphere. However, in Australia the red fox is a widespread exotic predator that has contributed to the decline and extinction of several native species. Here we describe a multiplex PCR assay for the molecular identification of the red fox. The identification is achieved by the generation of a diagnostic profile combining the lengths of mitochondrial ribosomal RNA (rRNA) gene regions amplified using highly conserved PCR primers. The method was tested in DNA samples from 17 species, including in mixtures. Our results demonstrate that the red fox has a unique combination of fragment lengths determined by capillary electrophoresis that can be used for its unambiguous discrimination from common domestic and wild species.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4