Assessment of the non-protein amino acid BMAA in Mediterranean mussel Mytilus galloprovincialis after feeding with estuarine cyanobacteria

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Mafalda S Baptista (Author) Other; Rita G W Vasconcelos (Author) Other; Paula C Ferreira (Author) Other; Marisa M R Almeida (Author) Other; Vitor M Vasconcelos (Author) Other

Journal

Title: Environmental Science and Pollution Research

Vol. 22 No. 9

Pages: 12501-12510

ISSN: 0944-1344

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 15 Citations

Other information

Authenticus ID: P-00G-3N3

DOI: 10.1007/s11356-015-4516-5

Abstract (EN): To determine whether 2-amino-3-methylaminopropanoic acid (BMAA) could be taken up by marine organisms from seawater or their diet mussels Mytilus galloprovincialis, collected from the North Atlantic Portuguese shore, were exposed to seawater doped with BMAA standard (for up to 48 h) or fed with cyanobacteria (for up to 15 days). Mussels were able to uptake BMAA when exposed to seawater. Mussels fed with cyanobacteria Synechocystis salina showed a rise in BMAA concentration during feeding and a decline in concentration during the subsequent depuration period. Cells from the gills and hepatopancreas of mussels fed with S. salina showed lessened metabolic activity in mussels fed for longer periods of time. A hot acidic digestion (considered to account for total BMAA) was compared with a proteolytic digestion, using pepsin, trypsin and chymotrypsin. The latter was able to extract from mussels approximately 30 % of total BMAA. Implications for BMAA trophic transfers in marine ecosystems are discussed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10