Involvement of mitochondrial proteins in calcium signaling and cell death induced by staurosporine in Neurospora crassa

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Gonçalves, A.P. (Author) Other; Cordeiro, J.M. (Author) Other; Monteiro, J. (Author) Other; Lucchi, C. (Author) Other; Paulo Correia de Sá (Author) Other; Arnaldo Videira (Author) Other

Journal

Title: Biochimica et Biophysica Acta - Bioenergetics

Vol. 1847

Pages: 1064-1074

ISSN: 0005-2728

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences

Other information

Authenticus ID: P-00G-C8D

DOI: 10.1016/j.bbabio.2015.05.011

Abstract (EN): Staurosporine-induced cell death in Neurospora crassa includes a well defined sequence of alterations in cytosolic calcium levels, comprising extracellular Ca2+ influx and mobilization of Ca2+ from internal stores. Here, we show that cells undergoing respiratory stress due to the lack of certain components of the mitochondrial complex I (like the 51 kDa and 14 kDa subunits) or the Ca2+-binding alternative NADPH dehydrogenase NDE-1 are hypersensitive to staurosporine and incapable of setting up a proper intracellular Ca2+ response. Cells expressing mutant forms of NUO51 that mimic human metabolic diseases also presented Ca2+ signaling deficiencies. Accumulation of reactive oxygen species is increased in cells lacking NDE-1 and seems to be required for Ca2+ oscillations in response to staurosporine. Measurement of the mitochondrial levels of Ca2+ further supported the involvement of these organelles in staurosporine-induced Ca2+ signaling. In summary, our data indicate that staurosporine-induced fungal cell death involves a sophisticated response linking Ca2+ dynamics and bioenergetics.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11