Use of liposomes to evaluate the role of membrane interactions on antioxidant activity

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Marlene Lucio (Author) FFUP; Helena Ferreira (Author) Other; Jose L F C Lima (Author) FFUP; Salette Reis (Author) FFUP

Journal

Title: Analytica Chimica Acta

Vol. 597 No. 1

Pages: 163-170

ISSN: 0003-2670

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 31 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-8RD

DOI: 10.1016/j.aca.2007.06.039

Abstract (EN): In this study the possibility of using liposomes as membrane mimetic systems was evaluated to estimate the antioxidant properties of oxicams and establish a relationship between the interactions of the drugs with the membrane and their consequent antioxidant activity. Different experiments were performed covering the study of the protective effect of oxicams in lipid peroxidation induced by the peroxyl radical (ROO center dot) derived from 2,2'azobis(2-amidinopropane) dihydrochloride (AAPH) and using two fluorescence probes with distinct lipophilic properties. Lipid peroxidation using the hydrophilic probe fluorescein was evaluated in lipid and aqueous media. Lipid systems labelled with the fluorescent probe diphenylhexatriene propionic acid (DPH-PA) were used to assess the effects of the drugs on membrane peroxidation simultaneously by fluorescence intensity decay and changes in membrane fluidity by steady-state anisotropy measurements. The use of different probes and liposomes as membrane mimetic systems allowed to conclude that membrane lipoperoxidation is related not only to the scavenging characteristics of the antioxidants but also to their ability to interact with the lipid bilayers.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8