Biological and physical-chemical characterization of phase pure HA and SI-substituted hydroxyapatite by different microscopy techniques

• Type: Article in International Scientific Journal
• Date: 2004

Authors

C. M. Botelho (Author) Other; R. A. Brooks (Author) Other; S. M: Best (Author) Other; Maria Ascensão Lopes (Author) FEUP; José Domingos Santos (Author) FEUP; N. Rushton (Author) Other; W. Bonfield (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: BIOCERAMICS, VOL 16

Vol. 254-2

Pages: 845-848

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 20 Citations

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

Other information

Authenticus ID: P-000-CBP

DOI: 10.4028/www.scientific.net/KEM.254-256.845

Abstract (EN): Two different microscopy techniques were used to investigate the response of human osteoblasts to hydroxyapatite (HA) and silicon substituted hydroxyapatite (Si-HA), namely, fluorescence and confocal microscopy The changes in the surface of HA and Si-HA, after incubation for different periods of time in simulated body fluid, were assed using atomic force microscopy and environmental electron scanning microscopy. Cell proliferation was higher on Si-HA compared to HA. In addition more focal points of adhesion were seen in Si-HA than on HA. Using atomic force microscopy and environmental scanning electron microscopy it was possible to observe changes in the surface of both materials, namely dissolution features and the formation of an apatite layer. These findings support the results of a previous study, which showed that Si-HA had a higher dissolution with the preferential release of silicon into the medium and this fact may account for the changes observed in the cell behaviour.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4