In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced hydroxyapatite plasma-sprayed coatings

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Ferraz, MP (Author) Other; Fernandes, MH (Author) FMDUP; Cabral, AT (Author) Other; Santos, JD (Author) FEUP; Monteiro, FJ (Author) FEUP

Journal

Title: Journal of Materials Science: Materials in Medicine

Vol. 10 No. 9

Pages: 567-576

ISSN: 0957-4530

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 25 Citations

Scopus - 27 Citations

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

Other information

Authenticus ID: P-001-3FZ

DOI: 10.1023/a:1008924516146

Abstract (EN): Human osteoblastic bone marrow cells were cultured for periods of up to 28 days in control conditions and on the surface of a glass reinforced hydroxyapatite composite (HA/G1) and commercial hydroxyapatite (HA) plasma-sprayed coatings, in the "as-received" condition and after immersion treatment in culture medium for 21 days. Cultures were characterized for total protein content and alkaline phosphatase activity. Scanning electron microscope analyses were performed on control cultures, seeded materials and materials incubated in the absence of cells. Culture media were analyzed for total and ionized calcium and phosphorus concentrations throughout the incubation period. Immersion of HA/G1 and HA coatings in culture medium resulted in significant alterations to the levels of calcium and phosphorus in the medium, leading to surface modifications. However, seeded material samples showed significant differences in the pattern of variation of the levels of these species. Cell proliferation was observed in the "as-received" HA/G1 composite, but cell mediated formation of mineral deposits was not proved. In contrast, "as-received" HA hardly supported cell growth. Previously immersed material samples showed cell proliferation and evidence of biological formation of mineral deposits. However, the HA/G1 composite presented better surface characteristics for cell growth as the behavior of bone marrow cells was closer to that observed in control cultures. (C) 1999 Kluwer Academic Publishers.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10