Lipid digestion, absorption and uptake in Solea senegalensis

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Pedro Borges (Author) Other; Francoise Medale (Author) Other; Vincent Veron (Author) Other; Maria dos Anjos Pires (Author) Other; Jorge Dias (Author) Other; Luisa M P Valente (Author) ICBAS

Journal

Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Vol. 166

Pages: 26-35

ISSN: 1095-6433

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 21 Citations

Scopus - 25 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-006-7ZT

DOI: 10.1016/j.cbpa.2013.05.007

Abstract (EN): Dietary lipids are the major energy source for metabolic purposes in most fish species, and improve dietary protein utilization for growth. In a previous study we have reported a low tolerance of Senegalese sole juveniles to dietary lipid levels and suggested a maximal dietary inclusion level of 8% lipids for both optimal growth and nutrient utilization. The mechanisms behind this apparent poor utilization of the dietary lipids are still to be elucidated. The primary aim of the present study was to investigate the overall process of digestion and lipid absorption in relation to dietary lipid levels. Triplicate groups of twenty fish (mean initial mass 29 g) were fed two isonitrogenous diets (54% of protein dry matter basis) with different lipid levels (L4 and L17, 4 and 17% lipids dry matter basis), for 88 days. Protein and lipid apparent digestibility coefficients as well as lipase activity were similar in both groups suggesting that Solea senegalensis has the ability to digest equally well a low fat or a high fat diet. Plasma triglyceride concentrations were significantly higher 5 and 16 h after feeding in fish fed the L17 compared to those fed L4, following dietary lipid supply, demonstrating effective lipid absorption. Expression of proteins related to lipid transport (microsomal triglyceride transfer protein), trafficking (Fatty acid binding protein 11) and fatty acid uptake (VLDL-r) was significantly higher in liver of fish fed the high fat diet 16 h after the meal, but remained unchanged in muscle. In conclusion, it seems that high fat diets do not impair lipid digestion and absorption.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10