Temperature affects methylation of the myogenin putative promoter, its expression and muscle cellularity in Senegalese sole larvae

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Catarina Campos (Author) Other; Luisa M P Valente (Author) ICBAS; Luis E C Conceicao (Author) Other; Sofia Engrola (Author) Other; Jorge M O Fernandes (Author) Other

Journal

Title: Epigenetics

Vol. 8

Pages: 389-397

ISSN: 1559-2294

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 28 Citations

Scopus - 52 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-006-CAA

DOI: 10.4161/epi.24178

Abstract (EN): Myogenin (myog) encodes a highly conserved myogenic regulatory factor that is involved in terminal muscle differentiation. It has been shown in mammals that methylation of cytosines within the myog promoter plays a major role in regulating its transcription. In the present study, the Senegalese sole (Solea senegalensis) myog putative proximal promoter was identified and found to be highly conserved among teleosts. Therefore, it is plausible that it plays a similar role in controlling myog expression. Cytosine methylation of the myog promoter in skeletal muscle of Senegalese sole larvae undergoing metamorphosis was influenced by rearing temperature. A lower temperature (15 degrees C) significantly increased myog promoter methylation in skeletal muscle, particularly at specific CpG sites, relatively to higher rearing temperatures (18 and 21 degrees C). Myog transcription was downregulated at 15 degrees C, whereas expression of dnmt1 and dnmt3b was upregulated, consistently with the higher myog methylation observed at this temperature. Rearing temperature also affected growth and fast muscle cellularity, producing larger fibers at 21 degrees C. Taken together, our data provide the first evidence of an epigenetic mechanism that may be underlying the temperature-induced phenotypic plasticity of muscle growth in teleosts.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9