Identification of carbapenem-resistant Acinetobacter baumannii clones using infrared spectroscopy

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Clara Sousa (Author) Other; Filipa Grosso (Author) Other; Luis Meirinhos Soares (Author) Other; Luisa Peixe (Author) FFUP; Joao Lopes (Author) Other

Journal

Title: Journal of Biophotonics

Vol. 7

Pages: 287-294

ISSN: 1864-063X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 27 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-009-H6B

DOI: 10.1002/jbio.201200075

Abstract (EN): In this work we assessed the discriminatory ability of Fourier-transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem-hydrolyzing class D -lactamases (CHDL)-producing Acinetobacter spp. (5 hospitals; 2001-2008) previously characterized by DNA-based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL-producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying bla(OXA-58), ST98 carrying bla(OXA-24/40)and ST92 carrying bla(OXA-23). Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8